Effects of olfactory ensheathing cells transfected with ciliary neurotrophic factor mediated by adenovirus intraocular transfer on intracranial optic nerve injury

Abstract

Objective To investigate the repairment effects of olfactory ensheathing cells (OECs) transfected with ciliary neurotrophic factor(CNTF) mediated by adenovirus intraocular transfer on intracranial optic nerve injury in rats.Methods Intracranial optic nerve injury model of rats were established by the microsurgery through subfrontal approach and the rats were averagely divided into different groups.The flash visual evoked potential(F-VEP) of different groups were observed at different time points after injury and the numbers of retinal ganglion cells (RGCs) were retrogradely labeled by fluorogold.Results In F-VEP test,the P1 wave amplitude of rats post injury decreased and latency increased (P ＜ 0.05).The P1 wave amplitude of olfactory ensheathing cells transfected with CNTF mediated by adenovirus group was higher than that in the control group and the latency was shorter meantime,there was significant difference (P ＜ 0.01).The numbers of labeled retinal ganglion cells of olfactory ensheathing cells transfected with CNTF mediated by adenovirus group was significantly higher than that in the control group (P ＜ 0.01).Conclusions The transfer of olfactory ensheathing cells transfected with CNTF mediated by adenovirus intraocular on rats after intracranial optic nerve injury can improve the repairment of visual function during early period. Key words: Ciliary neurotrophic factor; Olfactory ensheathing cells; Optic nerve injury