Effects of Nutrient Media on the In Vitro Establishment of Shoots of Pistacia Atlantica Desf. Seedling.

Abstract

Shoot cultures of Pistacia atlantica Desf. seedlings were established in vitro using apical and nodal segments from six-month-old plants. The most satisfactory results were obtained using a modified Quirin and Lepoivre (1977) macroeement solution identified as QL. 4 which was enriched with 10 mEq/I of NH4NO_ plus 5 mEq/l of NaNO3 and supplemented with MS (1962) micronutrients plus 0.4 mg/l thiamine-HCl, 0.4 mg/l pyridoxine-HCl, 100 mg/l m-inositol, 10-6 M BAP, 5x10-8 M NAA, 30 g/l sucrose and 6 g/l Difco-Bacto agar. All cultures were incubated 24ºC under a 16 h photoperiod (30 \alphaE/m2xs).