Effects of nonylphenol on the brain development-associated gene expression profiles of F1 generation rats

Abstract

To explore the effects of nonylphenol on brain development-associated gene expression profiles of F1 generation rats by means of microarray technique. The mRNA were extracted respectively from the brain of 2-day-old F1 generation rats whose F0 male generation were tested with and without nonylphenol, respectively, then reversely transcribed to cDNA and labeled with cy5 and cy3 fluorescence. Subsequently,the cDNA probes were hybridized to the Mouse40S cDNA microarray and the fluorescent signals of cy5 and cy3 were scanned and analyzed. Sixteen identified genes expressed differently, including 13 down-regulated in which five were related to brain function and development. Nonylphenol can effect the brain function in many ways, mainly disturb metabolism, development and differentiation of neurocyte, and synthesis and release of neurotransmitter.