Abstract
Regeneration of injured peripheral nerves is an extremely complex process. Nogo-A (neurite outgrowth inhibitor-A) inhibits axonal regeneration by interacting with Nogo receptor in the myelin sheath of the central nervous system (CNS). The aim of this study was to investigate the effects of Nogo-A and its receptor on the repair of sciatic nerve injury in rats. Sprague-Dawley rats (n=96) were randomly divided into 4 groups: control group (control), sciatic nerve transection group (model), immediate repair group (immediate repair), and delayed repair group (delayed repair). The rats were euthanized 1 week and 6 weeks after operation. The injured end tissues of the spinal cord and sciatic nerve were obtained. The protein expressions of Nogo-A and Nogo-66 receptor (NgR) were detected by immunohistochemistry. The protein expressions of Nogo-A, NgR, and Ras homolog family member A (RhoA) were detected by western blot. At 1 week after operation, the pathological changes in the immediate repaired group were less, and the protein expressions of Nogo-A, NgR, and RhoA in the spinal cord and sciatic nerve tissues were decreased (P<0.05) compared with the model group. After 6 weeks, the pathological changes in the immediate repair group and the delayed repair group were alleviated and the protein expressions decreased (P<0.05). The situation of the immediate repair group was better than that of the delayed repair group. Our data suggest that the expression of Nogo-A and its receptor increased after sciatic nerve injury, indicating that Nogo-A and its receptor play an inhibitory role in the repair process of sciatic nerve injury in rats.
Highlights
In recent years, central sensitization has been reported under recognized neuropathic conditions, there is a lack of information on the acute brain activation pattern of peripheral nerve injury [1]
H&E staining As shown in Figure 1A, there was no inflammatory cell infiltration at each time point in the control group, and the Antibody b-actin Nogo-A Nogo-66 receptor (NgR) Ras homolog family member A (RhoA) IgG
The expression of Nogo-A protein in the spinal cord and sciatic nerve tissues increased after sciatic nerve transection
Summary
Central sensitization has been reported under recognized neuropathic conditions, there is a lack of information on the acute brain activation pattern of peripheral nerve injury [1]. Peripheral nerve injury is a common clinical disease that causes the partial loss of segmental movement and sensory and autonomic nervous function, placing a heavy burden on patients and their families [2]. Increased attention has been paid to the treatment of sciatic nerve injury. The injury of sciatic nerve is related to the change of gene expression level [3]. The role of these up-regulated or downregulated genes remains unclear. Understanding the molecular mechanism of the occurrence and development of sciatic nerve injury is a prerequisite for the development of effective treatment for this highly prevalent disease
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