Effects of nitrogen dioxide on growth and yield of black turtle bean ( Phaseolus vulgaris L.) cv. ‘Domino’

Abstract

Twenty-six-day-old black turtle bean cv. ‘Domino’ plants were exposed to nitrogen dioxide (0·0, 0·025, 0·05 and 0·10 μl liter −1), 7 h per day for 5 days per week for 3 weeks, under controlled environment. Data were collected on net photosynthesis rate (PN), stomatal resistance (SR), and dark respiration rate (DR), immediately after exposure, 24 h after the termination of exposure and at maturity (when the leaves had just started turning yellow), using a LICOR 6000 Portable Photosynthesis System. Chlorophyll- a(Ch- a), chlorophyll- b (Ch- b), total chlorophyll (tot-Ch) and leaf nitrogen were measured immediately after exposure and at maturity. Growth characteristics—relative growth rate (RGR), net assimilation rate (NAR), leaf area ratio (LAR) and root: shoot ratio (RSR)—were computed for treated plants. Net photosynthesis rate increased by 53% in 0·10 μl liter −1 NO 2 treated plants immediately after exposure compared to control plants. Dark respiration rates were also higher in treated plants. Ch- a, Ch- b and tot-Ch showed significant increases with 0·1 μl liter −1 NO 2 treatment immediately after exposure. Foliar nitrogen content showed an increase in treated plants both immediately after exposure and at maturity. Increases were also seen in RGR and NAR. Plant yield increased by 86% (number of pods), 29% (number of seeds) and 46% (weight of seeds), respectively. Nitrogen dioxide stimulated the overall plant growth and crop yield.