Abstract

Abstract Studies concerning the use of the nitrification inhibitor, 2-chloro-6-(trichloromethyl)pyridine (nitrapyrin), have indicated that at least one site of action of nitrapyrin is associated with leaf chloroplasts. Leaves from radish (Raphanus sativus L. cv. Cherry Belle) grown with 10 ppm nitrapyrin in combination with 6 nitrogen fertilizers exhibited symptoms of nitrapyrin toxicity which ranged from reduced leaf size to marginal chlorosis and chlorotic mottling of leaves. The incorporation of nitrapyrin with nitrogen fertilizers, particularly organic sources of N, significantly reduced the number of chloroplasts per cell and significantly altered the structural integrity of radish chloroplasts relative to mesophyll cells and chloroplasts from plants grown at 0 ppm nitrapyrin.

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