Abstract
ObjectiveThe purpose of this study is to assess the effectiveness of the topical application of cacicol regenerating agent (RGTA) in an experimental model of corneal ulcer after photorefractive keratectomy (PRK) in mice. MethodsMice were subjected to PRK surgery with a 2.0mm ablation zone on the central cornea and 45mm of depth on a VISX Star S2 excimer laser. Corneas were treated topically with cacicol drops 1h and 48h after injury. Control groups received balanced salt solution (BSS) in the same dosage. Clinical and histopathological events were evaluated at 1, 2, 3 and 7 days after surgery.Sections obtained through the central region of the corneas were used to analyse the histopathological events of injured and healed corneas. α-SMA (myofibroblast transformation), E-cadherin (assembly of epithelial cells) and neuronal class III β-tubulin (innervation) were performed. ResultsCorneas treated topically with cacicol for 7 days showed a greater degree of transparency compared to controls. Cacicol treated corneas showed improved epithelial cytoarchitecture. Analysis of α-SMA profiles in the stroma showed that cacicol reduced or delayed the presence of myofibroblasts in the stroma compared to BSS (p<0.001). Finally, a putative neuroregenerative effect of cacicol was found in corneas subjected to an experimental PRK lesion. In some cases some interindividual variability could be observed due to the design of the experimental model. This is a limitation to consider, despite the statistical significance of the data. ConclusionsIn a model of laser induced surgical lesions in the cornea, topical application of an RGTA (i.e. cacicol) could be involved in avoiding myofibroblast scarring formation and promoting nerve regeneration.
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