Effects of nerve stimulation on amphibian oviductal activity

Abstract

The present study describes, for the first time in an anuran amphibian, the nerve stimulation effects on the secretory and motor activity of the oviduct of adult females. The results reveal that in Rhinella arenarum oviducts, the epithelial and glandular secretory cells of the mucosa of the pars convoluta respond to nerve stimulation secreting the products synthetized and stored in their cytoplasm. The ultrastructural analysis showed that the cell content released is made up of granular, fibrillar and floccular material, exocytosis being the main secretory mechanism found in epithelial secretory cells, although apocrine and holocrine processes could also be observed. In contrast, in glandular cells only exocytosis processes were found. With respect to the participation of the nervous system in the motility of the duct, observations under our experimental conditions indicated that oviductal nerve stimulation promotes motor activity as manifested by a succession of coordinated contractions and relaxations that generate movements similar to peristaltic waves. These results were observed in oviducts from animals captured during the reproductive and post reproductive periods. However, it is important to note that both the secretory response and duct motility are markedly decreased during the post reproductive period of the species.