Effects of Nanoherbal Haramonting (Rhodomyrtus tomentosa) and Extra Virgin Olive Oil on Histology of Liver and Kidney of Preeclamptic Rats.

Abstract

Preeclampsia (PE) is a life threatening disease prevailing in pregnant women in Indonesia. Impaired kidneys and livers function in PE has a high risk during pregnancy. This study aimed to determine the role of nanoherbal haramonting and Extra Virgin Olive Oil (EVOO) on kidney and liver safety in preeclampsia rats. Nanoherbal Rhodomyrtus tomentosa is a medicinal plant with antioxidant activity such as EVOO which acts as inhibitor of oxidation, reduces lipid peroxidation and increases the speed of epithelialization. This study used pregnant rats (Ratus norvegicus) consisting of 5 treatments. Animal models of preeclampsia were made by injection of NaCl 6% 3 mL/day/kg b.wt., subcutaneously in pregnancy of 6-12 days and then given herbs in pregnancy at 13-19 days. The study group consisted of, C-: Normal pregnant rats, C+: PE rats, T1: PE rats were given EVOO, T2: PE rats were given nanoherbal haramonting and T3: PE rats were given EVOO and nanoherbal haramonting. Tissue histology was made by the paraffin method and staining Hematoxylin Eosin which was dissected on the 20th day of pregnancy. There was no significant difference in weight of the liver and kidneys after administration of EVOO and nanoherbal haramonting. The administration of this herb significantly decreased the narrowing of the kidney tubules and the glomerular diameters. Both of these herbs also repaired preeclamptic liver damage (p<0.05) in normal hepatocyte cells, parenchymal degeneration and necrosis. The EVOO and nanoherbal haramonting repaired preeclamptic liver and kidney damage.