Abstract
Summary The effects of nanoencapsulated cumin essential oil (EO) feeding broilers on growth performance, mucin 2 gene expression, blood parameters, and immune responses to phytohemagglutinin (PHA) and 2, 4-dinitrochlorobenzene (DNCB) were evaluated. In a completely randomized design, 1050 male day-old Ross 308 broilers were distributed into 7 dietary treatments with 5 replicates of 30 birds per replication. Dietary treatments included 1) control basal (no additives), 2) control antibiotic growth promoter (flavophospholipol at 650 mg/kg), control basal plus a solution of 3) 150 mg/kg of chitosan nanoparticles, free-form cumin EO at 4) 100 or 5) 200 mg/kg, and nanoencapsulated cumin EO at 6) 100 and 7) 200 mg/kg. The use of 200 mg/kg nanoencapsulated cumin EO significantly (P
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