Effects of nangold on proliferation and apoptosis of human microvascular pericyte cells in vitro

Abstract

Objective To observe the effects of nangold(GNP) on proliferation and the expression of regulator of G-protein signaling5 (RGS5) of human microvascular pericytes.Methods The GNP solutions of 60 nmol/L with diameters of 25,50 and 100 nm were prepared.HBVP-1200 cells were cultured in vitro.The methyl thiazolium tetrazolium (MTT) was used to detect the effects of ditferent diameters of GNP on proliferation of pericytes.Atomic force microscope (AFM) was used to observe morphological changes of pericytes.The expression of RGS5 protein was detected by using Western blotting.Results GNP could euhance the proliferation of human microvascular pericytes.After treatment with different diameters of GNP (25,50,and 100 nm),the proliferation rate of pericytes was ( 147.9 ± 5.9) ％,( 121.7 ± 3.4) ％ and ( 107.6 ± 2.1 ) ％ respectively.When the diameter of GNP was smaller,the proliferation rate was increased significantly (P ＜0.05).Under AFM,the morphological changes of pericytes treated with GNP were observed,including invagination,obvious shrinked cell membrane and much rougher surface.The protein expression of RGS5 was reduced when the GNP was given 24 h later,especially when the diameter of GNP was 25 nm.Conclusion GNP could enhance the proliferation of human microvascular pericytes,and restrain the RGS5 protein expression.The smaller the diameter of GNP,the stronger effects of GNP on the pericytes. Key words: Gold nanoparticles; Pericyte; Regulator of G-protein signaling 5; Atomic force