Abstract
The effects of NaCl on the kinetic properties of desalted phosphoenolpyruvate carboxylase (PEP carboxylase, EC 4.1.1.31) from two halophytes, Suaeda monoica Forssk. ex. J.F. Gmel and Chloris gayana Kunth. were investigated. The tolerance of PEP carboxylase to NaCl in the reaction medium depends on the enzyme pre‐conditioning as well as on the concentration of its substrate PEP in the assay medium. Addition of PEP to the extraction and the storage medium, stabilizes the enzyme. Such a pre‐treated enzyme is inhibited by NaCl in the presence of low concentrations of PEP in the assay medium but is activated by NaCl in the presence of PEP at concentrations above 1.0 mM. NaCl modifies the nH value, K’ and Vmax, and seems to act as an allosteric effector.
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