Effects of N-benzyl-9-(2-tetrahydropyranyl) and indole-3-acetic acid in vitro culture of Butea buteiformis Voigt

Abstract

Butea buteiformis is a valuable tree with large trifoliate leaves with beautiful flower (Fig.1). It is distributed in tropical and subtropical region of Nepal. The seeds are bitter in taste and used for an anthelmintic. The plant possesses importance in agroforestry due to their nitrogen fixing capability. Plants are used for reforestration mostly on slope areas. The seeds were surface sterilized and cultured on half strength Murashige and Skoog (1962) (MS) medium. Nodal explants obtained from cultured were subcultured on different concentrations of N-Benzyl-9-(2-tetrahydropyranyl) (BPA) and Indole-3-acetic acid (IAA). The best proliferation of nodes and shoots were observed on the MS medium supplemented with 0.5 µM BPA and 0.5 µM IAA. After 8 weeks of culture the propagated plants were acclimatized and transferred to the sand box containing 1:1 soil and sand. Well rooted plants were then established in the field. All the data collected were worked out statistically with SPSS, a system of analytical procedure.