Abstract
Mouse peritoneal macrophages were prelabelled with [3H]AA and activated ex vivo with phorbol myristate acetate (PMA) in the presence or absence of added n-3 polyunsaturated fatty acids (n-3 PUFA). The n-3 PUFA did not affect the release of [3H]AA from stimulated macrophages. Added AA, enhanced, in a dose-dependent manner, the amount of free [3H]AA in the medium after activating the cells with PMA. Both n-3 PUFA and AA were actively acylated into the lipids of PMA activated macrophages, but the n-3 PUFA did not compete with reacylation of AA. In unstimulated cells, in the absence of PMA, n-3 PUFA effectively competed with AA for acylation into membrane phospholipids. These studies suggest that distinct pools of AA exist in macrophages.
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