Abstract
The aim of this study was to evaluate the effect of mineral trioxide aggregate (MTA) and Brazilian propolis on the cell viability, mineralization, anti-inflammatory ability, and migration of human dental pulp cells (hDPCs). The cell viability was evaluated with CCK-8 kit after 1, 5, 7, and 9 days. The deposition of calcified matrix and the expression of osteogenesis-related genes were evaluated by Alizarin Red staining and real-time PCR after incubation in osteogenic medium for 21 days. The expression of inflammation-related genes in cells was determined after exposure to 1 μg/mL LPS for 3 h. Finally, the numbers of cells that migrated through the permeable membranes were compared during 15 h. Propolis and MTA significantly increased the viability of hDPCscompared to the control group on days 7 and 9. In the propolis group, significant enhancement of osteogenic potential and suppressed expression of IL-1β and IL-6 was observed after LPS exposure compared to the MTA and control groups. The number of migration cells in the propolis group was similar to that of the control group, while MTA significantly promoted cell migration. Propolis showed comparable cell viability to that of MTA and exhibited significantly higher anti-inflammatory and mineralization promotion effects on hDPCs.
Highlights
Submitted: Noember 20, 2018 Accepted for publication: September 22, 2019 Last revision: November 8, 2019Pulpotomy is the most common method for preservation of the vital pulp of primary teeth or immature permanent teeth and is the preferred procedure when only the coronal pulp is inflamed due to bacterial penetration following carious, traumatic, or iatrogenic causes, and the radicular pulp is free from inflammation
Toxicity of materials human dental pulp cells (hDPCs) viability was slightly higher in 10 μg/mL
Both materials showed comparable cell viability to that of the negative control group (p>0.05, Figures 1A and B). 10 μg/mL of ethanolic extract of Brazilian propolis (EEP) and 1:8 dilution of Mineral trioxide aggregate (MTA) extraction were used in the subsequent experiments
Summary
Pulpotomy is the most common method for preservation of the vital pulp of primary teeth or immature permanent teeth and is the preferred procedure when only the coronal pulp is inflamed due to bacterial penetration following carious, traumatic, or iatrogenic causes, and the radicular pulp is free from inflammation. Mineral trioxide aggregate (MTA) was introduced in the early 1990s1 and has been successfully used in the endodontic field for perforation repair, root-end filling, one-visit apexification, and pulp capping in recent years. The clinical success of MTA is based on its superior sealing ability,[2] biocompatibility, and potential to induce odontoblast differentiation.[3] MTA – which has been the gold standard4 – was used as conventional pulp capping material Despite these advantages, MTA still has some drawbacks
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