Abstract
Objective To investigate the effects of MT1E gene on the cell proliferation in human pancreatic carcinoma cell line SW1990.Methods Human pancreatic carcinoma cell line SW1990 made by transfection with expressible MT1E gene, Positive monoclone was screened by G418.Western blotting assays were used to identify the expression of MT1E protein respectively. The cell proliferation was determined by MTT assay. The cell cycle was detected by flow cytometry, The apoptosis-related protein levels of Cyclin Dl and p53 determined by immunohistochemistry. Results MT1E protein were expressed in SW1990.Cells tranfected pcDNA3.1 ( - ) -MT1E compared with the SW1990 and cells tranfected pcDNA 3.1 ( - ), proliferation rate significantly enhanced, cells number of G0/G1 phase obviously decreased while cells number of S phase increased, Cyclin Dl and p53 protein levels elevated. Conclusion MT1E could promote proliferation of SW1990 cell by upregulating expression of cyclin Dl and p53 to facilitate cells into S phase. Key words: Pancreatic carcinoma; MT1E; Cyclin Dl; p53
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