Abstract
Objective To investigate the effects of morphine on PTEN expression and NF-kB activity in human gastric carcinoma cell line MGC-803. Methods The human gastric cancer cell line MGC-803 was purchased from Cell Biology Research Institute, Chinese Academy of Sciences, and cultured in DMEM liquid culture medium. The cells were randomly divided into 2 groups （ n = 6 each） ： control group and morphine group. The ceils was exposed to 0.1 μmol/L morphine in morphine group. The apoptosis was assessed by flow cytometry after being incubated with morphine for 24 h. PTEN expression and NF-kB activity were detected using RT-PCR and Western blot. Results The apoptotic rate was significantly increased, PTEN expression was up-regulated and NF- kB activity was significantly decreased in morphine group compared with control group （ P 〈 0.05）. Conclusion Morphine can promote the apoptosis in human gastric cancer cells by up-regulating PTEN expression and decreasing NF-kB activity. Key words: Morphine ; Stomach neoplasms ; Apoptosis ; PTEN phosphohydrolase ; NF-kappa B
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