Effects of Mollugo pentaphylla extract on monosodium urate crystal-induced gouty arthritis in mice

Yun Mi Lee,Dong-Seon Kim,Eun-Jung Shon,Ohn Soon Kim

doi:10.1186/s12906-017-1955-1

Yun Mi Lee, Dong-Seon Kim + Show 2 more

Open Access

https://doi.org/10.1186/s12906-017-1955-1

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Abstract

BackgroundGout is an inflammatory condition induced by the deposition of monosodium urate (MSU) crystals in joints and soft tissues, and it can lead to acute or chronic arthritis. MSU are pro-inflammatory stimuli that can initiate, amplify and sustain an intense inflammatory response. In this study, we evaluated the anti-inflammatory effect of an extract of Mollugo pentaphylla (MPE) on MSU-induced gouty arthritis in a mouse model.MethodAn MSU crystal suspension (4 mg/50 μL) was injected intradermally into the right paw. The mice were orally administered MPE (150 mg/kg or 300 mg/kg) or the positive control drug colchicine (1 mg/kg) 1 h before the MSU crystals were injected and then once daily for 3 days. The effects of MPE included inflammatory paw edema and pain upon weight-bearing activity, and we evaluated the inflammatory cytokine expression and paw tissue inflammation-related gene expression.ResultsMPE suppressed inflammatory paw edema and pain in the MSU-induced mice. MPE showed anti-inflammatory activity by inhibiting the production of TNF-α, interleukin (IL)-1β, NLRP3 inflammasome and NF-κB.ConclusionThese results suggest that MPE has potent anti-inflammatory activities and may be useful as a therapeutic agent against gouty arthritis.

Highlights

Gout is an inflammatory condition induced by the deposition of monosodium urate (MSU) crystals in joints and soft tissues, and it can lead to acute or chronic arthritis
Mollugo pentaphylla (MPE) showed antiinflammatory activity by inhibiting the production of Tumor necrosis factor-alpha (TNF-α), interleukin (IL)-1β, NLRP3 inflammasome and Nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). These results suggest that MPE has potent anti-inflammatory activities and may be useful as a therapeutic agent against gouty arthritis
And b, the MSU crystals in the treatment mice led to a significant increase in the foot thickness compared with that of the control mice; the increase in foot thickness was found to be reduced in the MSU crystal-induced mice treated with MPE (300 mg/kg) and colchicine. These results suggest that MSU crystal-induced paw edema was suppressed by MPE

Summary

Introduction

Gout is an inflammatory condition induced by the deposition of monosodium urate (MSU) crystals in joints and soft tissues, and it can lead to acute or chronic arthritis. Gout is characterized by increased blood uric acid levels (hyperuricemia) and the deposition of monosodium urate (MSU) crystals within intra-and/or peri-articular areas, which leads to excruciating pain and inflammatory processes [1]. Pre-treatment with intravenous colchicine before intra-articular MSU crystal injections greatly reduces inflammation, suggesting that colchicine targets the initial phase of inflammation [9, 10]. These agents may have serious side effects, such as gastrointestinal toxicity, renal toxicity, or gastrointestinal bleeding.

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