Abstract
AbstractWe investigated the cytotoxic effects of mitomycin C with two different vehicles, sodium chloride and mannitol, on the corneal endothelium. The corneal endothelia of paired rabbit and human corneas were perfused with mitomycin C (0.5 mg/ml) and Balanced Salt Solution (BSS) Plus. Changes in corneal swelling and f-actin staining of the endothelial cytoskeleton were determined. Endothelial permeability measurements of human corneas and electron microscopy of the endothelium were performed.After exposure of mitomycin C with the vehicle sodium chloride for 10 (n = 8) and 30 (n = 5) min to human corneas, a maximal decrease in corneal thickness occurred of —66.0 ± 9.4 μm and - 107.20 ± 8.9 μm, respectively. A similar temporary deswelling effect was observed in the rabbit cornea. F-actin staining revealed disruption of the cytoskeleton. Endothelial permeability after 30 min (n = 3) mitomycin C exposure was similar for mitomycin C and BSS Plus. Transmission electron microscopy of mitomycin C-perfused corne...
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