Abstract
To investigate the effect of mitochondrial DNA deletion and OSW-1 on PI3K-AKT signaling pathway PCR Array in SK-Hep1 hepatocellular carcinoma cells, we prepared SK-Hep1 cells with mtDNA deletion, that is, p0SK Hep. Then the OSW-1 of 100 ng/L was used to intervene SK-Hep1 and p0SK-Hep1. RT-qPCR was used to detect the difference of gene expression on PI3K-AKT signaling pathway PCR Array in four groups of cells. The gene expression of TLR4, FOS and TSC2 markers in SK-Hep1 cells treated with OSW-1 were significantly increased. The gene expressions of PDPK1, GJA1, TLR4 and TSC2 markers were significantly increased in p0SK-Hep1 cells, and the gene expressions of IRAK1 and GJA1 markers were significantly increased in p0SK-Hep1 cells treated with OSW-1. OSW-1 mainly affects the genes related to TLR4 pathway on PI3K-AKT signaling pathway PCR Array in SK-Hep1 HCC cells. P0SK-Hep1 mainly affects the upstream PDK1 gene and downstream TSC2 gene of Akt on PI3K-AKT signaling pathway PCR Array, and also affects the gene expression of gap junction at the same time.
Highlights
Phosphatidylinositol-3 kinase (PI3K)-mediated signal transduction pathway is closely related to the occurrence and development of malignant tumors and plays an important role in the proliferation, migration and metabolism of tumor cells [1]
The gene expressions of PDPK1, GJA1, TLR4 and TSC2 markers were significantly increased in p0SK-Hep1 cells, and the gene expressions of IRAK1 and GJA1 markers were significantly increased in p0SK-Hep1 cells treated with OSW-1
OSW-1 mainly affects the genes related to TLR4 pathway on PI3K-AKT signaling pathway PCR Array in SK-Hep1 hepatocellular carcinoma (HCC) cells
Summary
Phosphatidylinositol-3 kinase (PI3K)-mediated signal transduction pathway is closely related to the occurrence and development of malignant tumors and plays an important role in the proliferation, migration and metabolism of tumor cells [1]. 3β, 16β, 17α-trihydroxycholest-5-en-22-one16-O-(2-O-4-methoxybenzoyl-β-Dxylopyran osyl)-(1 → 3)-(2-O-acetyl-α-L-arabinopyranoside) (OSW-1) inhibits the growth of tumor cells in the aspects of cell invasion, angiogenesis, cell polarity and cell adhesion, affects the core genes in each signaling pathway, and induces apoptosis of hepatoma cells [4]. We prepared SK-Hep cells with mitochondrial DNA deletion, that is, p0SK-Hep, used real-time quantitative PCR(RT-qPCR) and detected the expression of PI3K-AKT signaling pathway PCR Array in order to clarify the effects of OSW-1 and mitochondrial DNA deletion on PI3K signaling pathway in hepatoma cells. The new tablets include members of AKT (protein kinase B) and PI3K family and their regulatory genes. These genes are involved in many biological processes: Gsk inactivation, B-catenin deposition, actin assembly regulation and cell migration, BAD phosphorylation and so on. Genes related to IGF-1, mTOR and anti-apoptosis signaling pathway are included
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