Abstract
BackgroundA growing body of evidence indicates that aberrant expression of miR-107 plays a core role in cancers. This study aims to demonstrate the function of miR-107 and its roles in chemo-drug resistance in breast cancer cells.MethodologyCCK-8 assays were carried out to test the effect of miR-107 mimics on the proliferation of MCF-7 cells. The apoptosis level of each group was detected by flow cytometry. miR-107 level, mRNA levels of Bcl-2/Bax and TRIAP1 were detected by quantitative real-time Polymerase Chain Reaction (qRT-PCR) analysis. Protein levels of Bcl-2/Bax, p-Akt/Akt in MCF-7 cells were detected by using Western Blot. Lastly, the dual luciferase reporter gene assay system was used to confirm interaction between miR-107 and its target gene TRIAP1.ResultsCCK-8 assays indicated that miR-107 mimics augmented Taxol-induced cell viability inhibition. Flow cytometry showed that miR-107 mimics augmented Taxol-induced elevation of cell apoptosis. qRT-PCR analysis revealed that miR-107 mimics inhibited the mRNA expression of Bcl-2 and induced the mRNA level of Bax. Western Blotting indicated that miR-107 mimics inhibited the expression of proteins Bcl-2 and p-Akt, and induced the expression of Bax, while showing no significant effects on Akt. The relative luciferase activity revealed that oncogene TRIAP1 is a potential target gene of miR-107.ConclusionsmiR-107 plays a role in regulating chemo-drug sensitivity in mammary cancer cell by targeting TRIAP1.
Highlights
Breast cancer remains a public-health issue on a global scale in women mainly aged between 20 to 59 years [1]
Flow cytometry showed that miR-107 mimics augmented Taxol-induced elevation of cell apoptosis. quantitative realtime Polymerase Chain Reaction (qRT-PCR) analysis revealed that miR-107 mimics inhibited the mRNA expression of Bcl-2 and induced the mRNA level of Bax
Conclusions: miR-107 plays a role in regulating chemodrug sensitivity in mammary cancer cell by targeting TP53-regulated inhibitor of apoptosis 1 (TRIAP1)
Summary
Breast cancer remains a public-health issue on a global scale in women mainly aged between 20 to 59 years [1]. The main reason is chemoresistance of cancer cells [3]. Molecule-targeted treatments were extensively used in clinical settings [4]. As a new method of anti-tumor therapy, the molecule-targeted treatment of tumors has been demonstrated as effective [5]; this treatment has had some visible effects in clinical outcomes. The molecular mechanisms underlying the resistance of breast cancer cells to Taxol has not been fully elucidated. A growing body of evidence indicates that aberrant expression of miR-107 plays a core role in cancers. This study aims to demonstrate the function of miR-107 and its roles in chemo-drug resistance in breast cancer cells
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