Effects of miR-410-3p on proliferation and apoptosis of prostate cancer cells and its mechanism

Abstract

Objective To observe the effect of miR-410-3p on the proliferation and apoptosis of prostate cancer cells PC-3 and DU-145, and to explore its possible mechanism. Methods The miR-NC (control group) or miR-410-3p (experimental group) was transfected to prostate cancer cell lines PC-3 and DU-145. The effect of miR-410-3p on the proliferation of prostate cancer cells was detected by cell counting kit (CCK-8) and clone formation experiment. The effect of miR-410-3p on apoptosis was detected by flow cytometry. The target gene of miR-410-3p was predicted by microRNA.org target gene prediction software. Fluorescence real-time quantitative polymerase chain reaction (qPCR) and Western blot were used to detect the changes of VEGFC at mRNA level and protein level. The expression of p-Raf-1 and p-ERK1/2 protein was detected by Western blot. Results After transfection of miR-410-3p, the cell proliferation ability was significantly decreased(P<0.05), the number of clones formed was significantly decreased(P<0.05)and the apoptosis was significantly increased (P<0.01), the expression of VEGFC at mRNA and protein levels were significantly decreased(P<0.01). The expression of p-Raf-1 and p-ERK1/2 protein was significantly decreased. Conclusions miR-410-3p can inhibit the proliferation of prostate cancer cells and promote the apoptosis of prostate cancer cells. The possible mechanism is to interfere with the expression of VEGFC gene, which provides a new idea for molecular targeted therapy of prostate cancer. Key words: Prostatic Neoplasms; Cell Proliferation; Apoptosis; MicroRNAs