Effects of Mineral-Solubilizing Microorganisms on Root Growth, Soil Nutrient Content, and Enzyme Activities in the Rhizosphere Soil of Robinia pseudoacacia

Abstract

Background: Abandoned mining sites are becoming increasingly common due to anthropogenic activities. Consequently, external-soil spray seeding technology has attracted increasing attention as a strategy to remediate them. However, significant challenges remain that greatly inhibit the efficacy of such technologies, such as insufficient nutrients available for plants. Methods: For this study, we designed an experiment, which involved the addition of mineral-solubilizing microorganisms and R. pseudoacacia seedlings to the external-soil spray seeding (ESSS) substrate, and measured the soil nutrients, enzyme activities, and root growth of R. pseudoacacia. Results: First, the combination of certain mineral-solubilizing microorganisms with ESSS advanced its efficiency by increasing the availability of soil nutrients and soil enzymatic activities in association with R. pseudoacacia. Furthermore, the improvement of root growth of R. pseudoacacia was intimately related to soil nutrients, particularly for soil total nitrogen (TN) and total sulfur (TS). In general, the effects of the J2 (combined Bacillus thuringiensis and Gongronella butleri) treatment for soil nutrients, enzyme activities, and plant growth were the strongest. Conclusion: In summary, the results of our experiment revealed that these mineral-solubilizing microorganisms conveyed a promotional effect on R. pseudoacacia seedlings by increasing the soil nutrient content. These results provide basic data and microbial resources for the development and applications of mineral-solubilizing microorganisms for abandoned mine remediation.