Abstract
Hu, W., Li, T., Lu, M., Wu, L., Meng, X. and Tian, Y. 2014. Effects of microRNA-mediated IGF-1 gene silencing on telomerase activity and cell proliferation in deer antler cells. Can. J. Anim. Sci. 94: 417-425. This study aimed to assess deer antler cell proliferation and telomerase activity after transfection with IGF-1microRNA eukaryotic expression recombinants. Four pairs of pre-microRNA were designed and synthesized based on the IGF-1 mRNA sequence, cloned into the pcDNA6.2-GW/EmGFPmiR eukaryotic vector and transfected into deer antler cells. IGF-1 expression after transfection with recombinants was measured by Real-time PCR and Western blotting. Telomerase activity in deer antler cells was assessed by TRAP-PCR-EB. Finally, the effects of IGF-1 silencing on cell proliferation and cell cycle were determined by MTT assay and flow cytometric analysis, respectively. The oligonucleotide sequences for microRNA expression were successfully inserted, and IGF-1 expression levels were significantly decreased in ...
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