Effects of microRNA-138-5p on the migration and invasion of pancreatic cancer PANC- 1 cells by targeting vimentin

Abstract

Objective To explore microRNA- 138- 5p(miR- 138- 5p) expression in pancreatic cancer and its effects on pancreatic cancer cell migration and invasion,and its mechanism. Methods Quantitative real- time polymerase chain reaction(FQ- PCR)was used to examine the expression of miR- 138- 5p in pancreatic cancer cell lines and primary carcinoma tissues from human patients.Lentiviral vector containing miR- 138- 5p mimics(lv- miR- 138- m),or miR- 138- 5p inhibitor(lv- miR- 138- i)was used to either up- regulate or down- regulate miR- 138- 5p in PANC- 1 cells, respectively.MiR- 138- 5p impact on PANC- 1 cell line migration and invasion abilities by wound- healing and Transwell cell invasion assay. The predicted targeting of miR- 138- 5p on vimentin was acknowledged by a dual luciferase assay. Results Expression of miR- 138- 5p in 8 pancreatic cancer cell lines were 1 to 10 times lower than in normal pancreatic cells, expression in pancreatic cancer tissue than in adjacent low 52.99±10.62 times.Lv - miR- 138- m significantly inhibited the migration and invasion of PANC- 1 cells capability, compared with the negative control group were lower 1.27±0.03, 4.96±1.16 times. Lv- miR- 138- i can significantly enhance their migration and invasive ability, compared with the negative control group, respectively 3.23±0.71, 3.40±0.66 times. MiR- 138- 5p and vimentin (VIM)3'untranslated region(3'UTR)region may be complementary binding,inhibition of expression can reduce the invasiveness effect VIM promote cell lv- miR- 138- i induced, suggesting miR- 138- 5p at least in part through inhibition of pancreatic regulation play VIM cancer cell invasion role. Conclusion MiR- 138- 5p play an important role in pancreatic cancer cell migration and invasion and it directly targeting regulate the VIM inhibit pancreatic cancer cell invasion, which may be one of the mechanisms which play an inhibition of pancreatic cancer cell invasion. Key words: Pancreatic cancer; MicroRNA; Migration; Invasion; Vimentin