Abstract
ObjectiveTo study the influence of MicroRNA-10b on proliferation and invasion of human low metastatic lung cancer cell 95-C and its mechanism. MethodsLipofectamine MicroRNA-10b eukaryotic expression plasmid was transfected into 95-C. The experiment group was divided into blank control group, empty vector transfected group and MicroRNA-10b transfected group. Real time quantitative RT-PCR was used to detect the expression of MicroRNA-10b and KLF4mRNA expression. Proliferations of cells were detected by cell proliferation assay, invasion of the detected the cell Transwell experiments, the expression of KLF4 protein was detected in Western blotting cells. ResultsThe proliferation rate of MicroRNA-10b plasmid transfection group increased significantly after transfection, invasion and migration ability enhancement, by comparison, there are statistically significant differences in the blank control group and negative control group (P<0.05); the expression of MicroRNA-10b plasmid transfection group KLF4 protein decreased, the difference was statistically significant (P<0.05); reduce the expression of MicroRNA-10b plasmid transfection group KLF4mRNA, but no significant differences (P>0.05). ConclusionsMicroRNA-10b may promote proliferation and invasion of 95-C cells by down regulating the expression of KLF4 protein.
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