Effects of microplastics and food particles on organic pollutants bioaccumulation in equi-fugacity and above-fugacity scenarios

Abstract

Microplastics (MPs), as emerging contaminants, sorb organic pollutants from the environment or leach out additives, thereby altering the fate of co-existing pollutants to organisms. We chose equi-fugacity and above-fugacity concentrations of polychlorinated biphenyls (PCBs) as background contamination and plastic additive concentrations, respectively, to investigate the effects of MPs on PCB bioaccumulation; we compared the effects of MPs with those of food-borne particles (FBPs). Co-exposure to MPs and FBPs at both the equi-fugacity and above-fugacity PCB concentrations had no obvious toxic effects (ROS generation and cyp1a expression) on zebrafish. When the zebrafish were exposed to the equi-fugacity PCB concentrations, the PCB concentrations reached 177.7–400.5 ng/g after a 7-d uptake; the presence of MPs did not significantly enhance PCB bioaccumulation. The remaining PCB concentrations in the fish after a 4-d depuration were 58.4–125.1 ng/g; the effects of MPs were the same as those during the uptake period. However, at the above-fugacity PCB concentrations, the MPs markedly increased the PCB bioaccumulation (by 1.8–fold) to 712.9 ng/g. This is because at above-fugacity concentrations, PCBs on MPs migrate to organisms as there were high fugacity gradients. The FBPs enhanced PCB bioaccumulation in zebrafish more effectively than the MPs, even after depuration. In the presence of FBPs, PCB bioaccumulation increased by 2.8- and 4.2- fold after uptake in the equi-fugacity and above-fugacity scenarios, respectively, both of which were significantly higher than that observed for the MPs. This is probably because FBPs are easily assimilated by fish, making the associated PCBs more bioavailable. Finally, during the co-existence of MPs and FBPs, MPs facilitate the depuration of PCBs accumulated via FBP vectors; conversely, FBPs did not affect PCB accumulation via MP vectors. Thus, this study elucidated the effects of MPs and FBPs on the bioaccumulation of pollutants at equi-fugacity or above-fugacity concentrations in aquatic environments.