Abstract
Microplastics that are eaten by fish will bioaccumulate and will settle in the fish's body, especially in the gills and stomach organs. Exposure to microplastics in farmed animals can cause damage to gonadal cells and increase the level of aromatase in fish body so that the synthesis of androgens to become estrogene is higher. Cyp19b is a gene responsible for the synthesis of the aromatase enzyme which is related to the androgen hormone. The purpose of this study was to analyse the effect of giving polystyrene to catfish (C. gariepinus) feed on changes in the Cyp19b gene sequence. This research was descriptive experimental research. Results and observation data were analysed using a comparative descriptive method. The study was conducted by examining the brain of catfish (C. gariepinus) to determine changes in the Cyp19b gene sequence. The first step was to extract DNA from the gonads and fish brains, then examine the Cyp19b gene sequence. The next step was constructed a phylogenetic tree to determine if there is a change in kinship in the Cyp19b gene sequence. The results showed a trend towards changes in the Cyp19b gene sequence which can be described in a phylogenetic tree.
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