Abstract
The effects of glucocorticoids on renal and intestinal calcium binding protein (calbindin-D 28K and calbindin-D 9K) were examined in normal and uremic rats. Chronic uremic rats and normal controls were treated with either methylprednisolone (MP) 1.3 mg/kg/d or isotonic saline given as a continuous intraperitoneal infusion for 1 week before sacrifice. Renal calbindin-D 28K was measured by rocket immunoelectrophoresis and intestinal calbindin-D 9K was measured by an enzymelinked immunoadsorbent assay. Methylprednisolone treatment of chronic uremic rats increased plasma phosphate levels ( P < 0.05), but plasma calcium and 1, 25-dihydroxyvitamin D 3 were unchanged in all groups. MP treatment did not affect the renal calbindin-D 28K in either normal or uremic rats. In normal rats, MP treatment reduced intestinal calbindin-D 9K by 28% when compared to placebo ( P < 0.05). In contrast, chronic uremia increased renal calbindin-D 28K by 51% and 38% ( P < 0.001) in placebo and MP treated uremic rats, respectively, while intestinal calbindin-D 9K was unchanged. Thus, MP treatment and chronic uremia induced different changes in renal and intestinal calbindin-D of the rat suggesting that different mechanisms are involved in the regulation of these vitamin D dependent proteins.
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