Abstract
Fragile histidine triad (FHIT) is a new tumor suppressor gene, whose promoter methylation plays an important role in carcinogenesis. The aim of this study is to explore the effects of CpG island methylation on protein and mRNA expression of FHIT in non-small cell lung cancer, and the roles of FHIT gene in pathogenesis of NSCLC. Methylation and protein expression and transcriptional level of FHIT gene were detected by methylationspecific PCR, Western Blot and RT-PCR in 52 tumor tissues and normal tissues of NSCLC. Methylation in the tumor samples was detected at 38.46%, whereas it occurred at lower frequencies (7.69%) in the corresponding normal lung tissues. Protein expression frequencies was 28.8% in the tumor samples, whereas it was 88.5% in the corresponding normal lung tissues. MRNA expression frequencies was 51.9% in the tumor samples and 100% in the corresponding normal lung tissues. In NSCLC, FHIT gene promoter methylation frequency was significantly increased with decreased expression, suggesting that FHIT promoter methylation plays a role in lung cancer carcinogenesis.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
