Abstract
Elicitors have been widely used as biotic and abiotic stimulants in triggering the production of secondary metabolites in plant cell culture. The present study aimed to enhance the production of camphor and phenolic compounds and cell growth using methyl jasmonate (MeJA) and salicylic acid (SA) in the cell suspension culture of Turkish endemic species Achillea gypsicola. Various concentrations (0, 10, 50, and 100 μM) of MeJA and SA were applied to 8-day-old cell cultures. The camphor and phenolic compound contents were determined using a headspace gas chromatographic-mass spectrometer device and spectrophotometer. Increasing doses of MeJA and SA significantly enhanced the accumulation of the camphor and phenolic compounds in general. The highest amount of camphor accumulation occurred in cells treated with 100 μM MeJA (0.3449 μg/g) and 50 μM SA (0.3816 μg/g). Increasing concentrations of MeJA resulted in a significant decrease in the total anthocyanin when compared to the initial culture. The present study showed that MeJA and SA could effectively be used as potent elicitors to enhance the production of camphor and phenolic compounds, along with cell growth, in cell suspension cultures of the endemic Turkish yarrow species Achillea gypsicola.
Highlights
The potential benefits of medicinal and aromatic plants are largely accounted for by naturally occurring bioactive compounds, called secondary metabolites (Bourgaud et al, 2001)
Treatment with lower concentrations of methyl jasmonate (MeJA) (10 and 50 μM) decreased the total flavonol content when compared to the initial culture, but the treatment of 100 μM MeJA resulted in an increase, where its content was identical to that of the control
Achillea gypsicola is a perennial herbaceous-wooly plant that grows in steppe-limestone areas of central Anatolia (Akcin and Akcin, 2010)
Summary
The potential benefits of medicinal and aromatic plants are largely accounted for by naturally occurring bioactive compounds, called secondary metabolites (Bourgaud et al, 2001). In this respect, is of considerable importance and is widely used as an attractive alternative for producing valuable secondary metabolites (Georgiev et al, 2009; Wang et al, 2017). Plant cell culture has a challenge of low yield of plant secondary metabolites (Zhao et al, 2005). In vitro elicitorinduced production of secondary metabolites has recently gained considerable interest worldwide to overcome the low yield of such plant phytochemicals (Namdeo, 2007; Matkowski, 2008; Patel and Krishnamurthy, 2013; Dias et al, 2016)
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