Abstract
The effects of methyl jasmonate (MJ) and elicitor on the phenylpropanoid metabolism in tobacco was studied. It was found that suspended cells of tobacco, responded to MJ and elicitor from the plant pathogenic fungus Fusarium solani with differential formation of phenolic compounds. Both MJ and elicitor induced phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) activity to comparable levels. Northern hybridization showed that the increase in PAL activity was preceded by PAL mRNA accumulation. PAL was induced transcriptionally and enzymatically faster in elicitor-treated cells as compared to MJ-treated cells. HPLC of extracts from tobacco cells and their culture filtrate treated with MJ, showed an induction of the syntheses of coumarin-scopoletin and scopolin. This effect was not seen in elicitor-treated or control cell cultures. Scopolin was mainly found within the cell (99%), whereas scopoletin accumulated in the culture filtrate (90%). We conclude that though MJ mimics stress, indicating its possible role in the plant defense metabolism, responses of tobacco cells to MJ and elicitor are distinct.
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