Abstract
Objective: study aims to examine the effect of acetaminophen in male rats after early postnatal exposure to acetaminophen, on several behavioral outcomes and its role to precipitate the autistic like behavior with investigating the ameliorating role of melatonin on these parameters. Materials and Methods: Thirty healthy Albino male rat pups were selected for this study. At post natal day 3 (PND3), rat pups were divided into three groups (10 pups group). Group1were served as a control group were injected SC with NS for 7 days then gavaged with NS for 2 weeks , Group 2 pups which were injected SCin a dose (50 mg/kg) of acetaminophen once daily for 7 days then these pups were gavaged with NS for 2 weeks. Group 3 : pups which were injected SC in a dose (50 mg/kg) acetaminophen once daily for 7 days then gavaged with melatonin (10 mg/kg) for 2 weeks. behavioral test, such as nest seeking test at 9, the negative geotaxis test was done at PND 15 and 17, open field test at PND21 Results: acetaminophen exposed pups exhibited a significantly difference in behavioral tests: nest seeking test showed that the time of pups of acetaminophen group to approach maternal bedding was (10.00±1.51) sec which was significantly higher than time of control group. At PND15, NG test showed a significant increase in time spend to rotate 180° of the pups of acetaminophen group when compared with that of the control group and melatonin group. While at PND17, showed a significant increase in time spend to rotate 180° in NG test of pups of the acetaminophen group when compared with that of the control group and that of the melatonin treated group. open field test showed that pups of acetaminophen group were traveled significantly number of squares crossed (31±8.34) compared to those traveled by pups of control and melatonin treated groups (22±7.43).. Conclusion: Administration of acetaminophen from 3 to PND10 induces autistic like behavioral manifested by several alteration in rat pups. These alteration were ameliorated by administration with melatonin (10 mg/kg for 2 weeks) after exposure to acetaminophen.
Highlights
Administered with normal saline for 2 weeks orally
The present study demonstrated that acetaminophen exposed rat pups exhibited a significant difference in behavioural tests: nest seeking test showed that the pups of group 2 to approach the maternal bedding was (10.00±1.51) sec which was significantly higher than the time of the control group(4.50±2.19) sec
The present study aims to investigate the effect of melatonin on autistic-like behavior induction by acetaminophen and study the ameliorating effect of melatonin on behavioural alterations induced by acetaminophen
Summary
Group 2: pups injected acetaminophen subcutaneously in a dose (50mg/kg) once daily for 7 This is an open access article under the term of days, these pups were administrated with normal saline for 2 weeks. To view a copy of dose (50 mg/kg) once daily for 7 days administrated with melatonin (10 this license, visit: mg/kg) for 2 weeks Behavioural tests, such as nest seeking, were performed http://creativecommons.org/licenses/by/4.0/ at PND 9; negative geotaxis test was done at "PND 15 and 17", an open field test was done at PND21. While at PND17, pups showed a significant increase in time spend to rotate 180° in negative geotaxis test in group 2 (7.40±2.90) sec compared with that of the control group (3.12 ±1.33) sec, and that of the melatonin treated group These alterations were ameliorated by melatonin administration (10 mg/kg for 2 weeks) after exposure to acetaminophen
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