Abstract
Background: Low-dose X-rays are commonly used in medical imaging to help in the diagnosis ofdiseases. However, the deleterious effects of exposure to medical diagnostic low-dose X-rays remaina highly debated topic. The objective was to study the effects of medical diagnostic X-rays on humanblood cells.
 Materials and Methods: We studied the effects of medical diagnostic low-dose X-rays (80kVp), i.e.,0.01 or 0.05 mGy, after the in vitro exposure of human red blood cells (RBCs) and peripheralblood mononucleated cells (PBMCs).Cells with no irradiation served as the control group. The biologicalendpoints that were used to determine the effects of medical diagnostic low-dose X-rays were hemolysisfor RBCs and mitochondrial membrane potential, lysosomes, and the cell cycle for PBMCs.
 Results: Ourresults showed no changes in the hemolysis of RBCs and mitochondrial membrane potential, lysosome, orcell cycle in cells exposed to these low doses of X-rays when compared to the corresponding nonirradiatedcells at all harvest timepoints.
 Conclusion: These results suggested that there were no deleterious effectsof diagnostic low-dose X-rays when human RBCs and PBMCs were exposed in vitro.
 Bangladesh Journal of Medical Science Vol.20(1) 2021 p.136-144
Highlights
Exposure to high doses of radiation is known to have various harmful effects on cells or tissue 1-3
Similar to the mitochondrial membrane potential values, these data show no change in the mean acridine orange (AO) fluorescence values in irradiated peripheral blood mononucleated cells (PBMCs) when compared to the corresponding nonirradiated PBMCs at all harvest times
It is believed that a high dose of ionizing radiation contributes to cell damage,less is known about the cellular response to low doses of ionizing radiation, such as medical diagnostic X-rays.The present study showed the effects of 80 kVp medical diagnostic X-rays on human blood cells (RBCs and PBMCs)
Summary
Exposure to high doses of radiation is known to have various harmful effects on cells or tissue 1-3. There are several previous works have shown no harmful effects after in vitro or in vivo receiving to low-dose radiation[7,8,9,10,11,12,13,14,15,16,17,18]. The deleterious effects of exposure to medical diagnostic low-dose X-rays remain a highly debated topic. Materials and Methods: We studied the effects of medical diagnostic low-dose X-rays (80 kVp), i.e.,0.01 or 0.05 mGy, after the in vitro exposure of human red blood cells (RBCs) and peripheral blood mononucleated cells (PBMCs).Cells with no irradiation served as the control group. Conclusion: These results suggested that there were no deleterious effects of diagnostic low-dose X-rays when human RBCs and PBMCs were exposed in vitro.
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