Effects of mating dynamics and crowding on sex ratio variance in mice.

Abstract

Mating units of six virgin females and one adult stud male were established to test for the effects of timing of mating and crowding of pregnant females on litter sex ratios in mice. Females either copulated during periods when no other female of the mating unit copulated simultaneously (single mating condition) or when more than one female copulated (multiple matings condition). Two crowding conditions were imposed on the animals: the females of 14 mating units were placed into individual cages after mating (isolated condition), while females of the other 13 mating units remained in the original group until shortly before littering (crowded condition). Sex ratio variance did not deviate from random expectation in litters arising from the multiple matings periods. However, in litters arising from single mating periods, extreme sex ratios were found significantly less frequently than expected by chance. Higher sex ratio variance in litters arising from multiple matings periods is attributed to the timing of mating being at higher variance under this condition, which is known to affect sex ratios in other rodents. Crowding significantly reduced sex ratio variance further. Reduced sex ratio variance under single mating and crowded conditions is speculated to follow from competition for resources between preimplantation embryos, which may be further increased by stressful effects of crowding. Loss of embryos after implantation appeared not to be responsible for the above effects.