Abstract
Three experiments were conducted with everted gut sacs to investigate the effects of Mn source and Ca on Mn absorption in different intestinal segments of broilers. In Experiment 1, the Mn uptake by everted sacs of duodenum, jejunum, and ileum after different incubation times was compared to determine an optimum incubation time. In Experiment 2, 8 different Mn sources, including MnSO4, organic Mn sources (Mn-amino acid chelates, Mn-amino acids complexes with different complex strength), and mixtures of MnSO4 with Gly or Met were used to assess the effect of Mn source on Mn uptake. In Experiment 3, the effect of Ca in media on the uptake of Mn from MnSO4 and Mn-amino acids complexes was investigated. The uptake percentages of Mn by everted ileal sacs were significantly higher than those by duodenal and jejunal sacs. The uptake of Mn as Mn-Met chelate was significantly higher than that of Mn as Mn-Gly chelate. The uptake percentages of Mn as Mn-amino acid complex with moderate complex strength (Mn-AA A) and Mn-amino acid complex with strong complex strength (Mn-AA B) were significantly higher than that of Mn as MnSO4. The uptake of Mn as Mn-AA B by jejunal sacs was significantly higher than that of Mn as Mn-AA A in media containing high level of Ca. The uptake percentages of Mn at high Ca level were significantly higher than those at normal level. The results indicate that when incubated in vitro, ileum was the main site of Mn absorption for broilers. The absorption of Mn as organic sources were higher than that of Mn as inorganic Mn. The absorption of Mn as Mn-AA B with strong complex strength by jejunal sacs was higher than that of Mn as Mn-AA A with moderate complex strength in media containing high level of Ca. As a ligand, Met was more effective in facilitating Mn absorption than Gly. Furthermore, the transportation of Mn through intestinal cells was enhanced by adding Ca as CaCl2 to the buffer solutions.
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