Effects of manganese on fat snook Centropomus parallelus (Carangaria: Centropomidae) exposed to different temperatures

Bárbara Louise L Tuzuki,Caroliny P De Melo,Luciana N Ribeiro,Adriana Regina Chippari-Gomes,Frederico Augusto C Delunardo,Levy Carvalho Gomes

doi:10.1590/1982-0224-20170054

Bárbara Louise L Tuzuki, Caroliny P De Melo + Show 4 more

Open Access

https://doi.org/10.1590/1982-0224-20170054

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Journal: Neotropical Ichthyology	Publication Date: Dec 18, 2017
Citations: 14	License type: cc-by

Affiliation: Universidade Vila Velha

Abstract

ABSTRACT This study evaluates the effects of exposure to manganese (Mn2+) for 96 hours at two different temperatures (24 and 27°C) on juveniles of Centropomus parallelus through the activities of glutathione S-transferase (GST) and catalase (CAT), micronuclei test (MN) and comet assay. The GST activity did not show any significant difference between the groups exposed to Mn2+ and the respective control groups; in contrast, a major increase in the CAT activity was observed at 27°C in the group exposed to Mn2+ compared to the control group. The genotoxic analyses showed that in all animals exposed to Mn2+, the number of red cells with micronuclei increased significantly compared to the respective control groups. There was also a significant increase in the incidence of DNA damage in the groups exposed to Mn2+. At a temperature of 24ºC, animals exposed to Mn2+ had more DNA damage than those at 27°C. It is likely that the increase in temperature can also induce oxidative stress. Thus, we conclude that manganese is toxic to the fat snook juveniles, causing genotoxic damage, and when associated with an increase in temperature, manganese can also provoke an increase in oxidative stress.

Highlights

Global climate change is one of the critical challenges that can affect ecosystem health and chemical safety
Some glutathione S-transferase (GST) changes were expected in fish exposed to Mn2+ since GST plays a significant role in metabolism, acting on the detoxification of some electrophilic compounds, and changes in the activities of this enzyme directly reflect metabolic disturbances and cell damage in specific organs of fish (Carvalho-Neta, Abreu-Silva, 2013); this response was not verified in this study
These studies reported that the levels of GSH-dependent antioxidant enzymes, such as glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione S-transferase (GST), in the different tissues of Carassius auratus (Bagnyukova et al, 2007), Morone saxatilis (Grim et al, 2013) and Notothenia coriiceps (Machado et al, 2014) were slightly affected by the increase in temperature

Summary

Introduction

Global climate change is one of the critical challenges that can affect ecosystem health and chemical safety. Many aquatic environments are affected by the release of human contaminants, resulting in increased metal concentrations (Gomiero, Viarengo, 2014). These facts can lead to severe impairments to aquatic organisms because the rise in water temperature may modify the chemistry of many pollutants (Schiedek et al, 2007). These facts can lead to increases in the bioavailability of pollutants in the environment and, the metal uptake rates and their toxicity (Bervoets et al, 1996; Vergauwen et al, 2013; Lee et al, 2014)

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