Abstract
Objective:To evaluate the antitumor effects of polyethylene glycol (PEG) microspheres with adsorbed Hancornia speciosa ethanolic extract (HSEE) on blood mononuclear (MN) cells co-cultured with MCF-7 breast cancer cells.Methods:PEG microspheres were adsorbed with HSEE and examined by flow cytometry and fluorescence microscopy. MCF-7 and MN cells obtained from volunteer donors were pre-incubated alone or co-cultured (MN and MCF-7 cells) for 24 h with or without HSEE, PEG microspheres or PEG adsorbed with HSEE (PEG-HSEE). Cell viability, superoxide release and superoxide dismutase were determined. Results:Fluorescence microscopy showed that PEG microspheres were able to absorb HSEE throughout their surface. Irrespective of the treatment, the viability index of MN cells, MCF-7 and their co-culture was not affected. Superoxide release increased in co-cultured cells treated with HSEE, adsorbed or not onto PEG microspheres. In co-cultured cells, SOD levels in culture supernatant increased in the treatment with HSEE, adsorbed onto PEG microspheres or not. Conclusion:HSEE has direct effects on MN cells co-cultured with MCF-7 cells. The results suggest the benefits of Hancornia speciosa fruit consumption by women at risk of breast cancer. In addition, because PEG-HSEE maintained oxidative balance in co-cultured cells, it is a promising alternative for the treatment of tumor cells.
Highlights
Cancer is one of the most serious public health problems worldwide (Siegel et al, 2017)
A recent study found that a mixture of plant extracts adsorbed onto polyethylene glycol (PEG) microspheres have antitumor effects, and the authors suggested that the participation of blood mononuclear (MN) cells is important in achieving that result (Ribeiro et al, 2018)
Phosphate buffered saline (PBS); Polyethylene glycol (PEG) microspheres; H. speciosa ethanolic extract (HSEE); H. speciosa ethanolic extract adsorbed onto PEG microspheres (PEG-HSEE). *p
Summary
Cancer is one of the most serious public health problems worldwide (Siegel et al, 2017). In the search for cancer preventing drugs and treatments with few or no side effects, numerous studies have investigated medicinal plants (Greenwell and Rahman, 2015) combined with modified drug delivery systems such as a polymer matrix (Tran et al, 2011). PEG has been used as a carrier of different microparticulate drugs delivered in oral and parenteral systems developed to treat a number of diseases, including cancer (Gulapalliand-Mazitelli, 2015). The combination of medicinal plants and polymer matrices raises the possibility of developing new drugs and treatments. A recent study found that a mixture of plant extracts adsorbed onto PEG microspheres have antitumor effects, and the authors suggested that the participation of blood mononuclear (MN) cells is important in achieving that result (Ribeiro et al, 2018)
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