Effects of magnetite on anaerobic digestion of swine manure: Attention to methane production and fate of antibiotic resistance genes

Abstract

Effects of magnetite on methane production and fate of antibiotic resistance genes (ARGs) during anaerobic digestion (AD) of swine manure were investigated. Results showed that methane production was increased by maximum 16.1%, and magnetite could enhance the acetoclastic methanogenesis not hydrogenotrophic methanogenesis reflected by the functional gene quantification and microbial community analysis. The propionate degradation rate was improved, and it was syntrophic oxidized into H+/e−/CO2 for direct interspecies electron transfer (DIET) and acetate, where DIET was further enhanced by magnetite and the acetate was transformed into methane through syntrophic acetate oxidation (SAO) pathway. Magnetite mainly influenced the ARGs at the interim period of AD, where ARGs especially ermF were significantly enriched. Magnetite did not influence the total ARGs abundance at the end, although the tetM was enriched and mefA was reduced finally. Statistical analysis indicated that magnetite influenced the ARGs fate mainly through the changes of microbial community.