Abstract
Lycium barbarum has been used for more than 2500 years as a traditional herb and food in China. We investigated the effects of Lycium barbarum polysaccharides (LBP) on apoptosis, oxidative damage, and expression of adhesion molecules in bone marrow mononuclear cells (BMNC) of mice injured by ionizing radiation. Kunming mice were exposed to X-rays; then mice in the LBP groups were continuously injected with various concentrations of LBP intraperitoneally for 14 days. Mice in the control group were continuously injected with normal saline (NS) by the same route for 14 days. A normal group was set up. After 1, 7, and 14 days of treatment, mice were killed and BMNC were extracted. Cell cycle, apoptosis, and the expression of adhesion molecules CD44 and CD49d were detected by flow cytometry. The levels of malondialdehyde (MDA) and superoxide dismutase (SOD) were identified by colorimetric analyses. LBP significantly decreased the percentage of G0/G1 phase, apoptosis, MDA level, and expression of CD44 and CD49d and distinctly increased the activity of SOD. LBP showed a protective effect on BMNC against ionizing radiation-induced apoptosis and oxidative damage and altered the expression of adhesion molecule.
Highlights
The impact of ionizing radiation on humans is an increasingly serious issue
Recent studies show that Lycium barbarum polysaccharides (LBP) has a protective effect against ionizing radiation-induced damage; LBP can protect the reproductive function and prevent spermatogenic cell apoptosis induced by irradiation and enhance self-repair of the testis [2, 3, 23, 24]
P-Phycoerythrin- (PE-) antimouse Sca-1+ antibodies and isotype control antibody were obtained from eBioscience Corporation (Shanghai, China)
Summary
The impact of ionizing radiation on humans is an increasingly serious issue. The degree of damage to human health is closely related to the dose of radiation and duration of exposure. Lycium barbarum polysaccharide is the main component of these biological activities, with a relative molecular mass of 68–200 kDa [5]. Recent studies show that LBP has a protective effect against ionizing radiation-induced damage; LBP can protect the reproductive function and prevent spermatogenic cell apoptosis induced by irradiation and enhance self-repair of the testis [2, 3, 23, 24]. It can promote the immune function recovery of radiated mice [25]. LBP played an effective role in protecting bone marrow mononuclear cells against ionizing radiation injury
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