Effects of Luteolin on the Proliferation of Rat Lung Fibroblasts

Abstract

Aim To approach the effects of luteolin on proliferation of rat lung fibroblasts. Methods The lung fibroblasts were isolated from the lungs of Sprague-Dawley rats at postnatal day 3, and were then passaged to 96-well culture plates. All cells were divide into 6 groups including normal control group, positive control group, 4×10 time, the cytomorphology of LFb, drew growth curve, determined superoxide dismutase (SOD) activity were observed. Results Luteolin at the concentration of 4×10 −4, 4×10 −5 mol·L −1 inhibited evidently the growth and proliferation of lung fibroblasts, and enhanced SOD activity. The density, interval and volume of lung fibroblasts became decrescent. The effects, dose-dependently, had obvious statistical significance compared with normal control group. Conclusion Luteolin can inhibit the proliferation of the lung fibroblasts in rat.