Abstract
Vietnamese Sophora Root mainly contains active constituents such as alkaloids, and it has anti-tumour, antibacterial, and anti-inflammatory effects. The objective of the paper was to study the effects of Vietnamese Sophora Root on growth, adhesion, invasion and motility of mouse melanoma B16BL6 cells, and to preliminarily explore its mechanism of action. MTT assay was used to detect the effect of Vietnamese Sophora Root aqueous extract on B16BL6 cell proliferation. Cell adhesion assay, reconstituted basement membrane invasion assay and chemotactic motility assay were used to observe the effects of Vietnamese Sophora Root aqueous extract on adhesion, invasion and motility of B16BL6 cells. Different concentrations of Vietnamese Sophora Root aqueous extracts had different degrees of inhibitory effects on B16BL6 proliferation. With the decrease of concentration, the proliferation inhibitory effect decreased and even turned to promoting effect. The extract significantly inhibited the adhesion of B16BL6 cells to the basement membrane component LN, and had a significant effect on both the invasive and migratory capacities of B16BL6 cells through the basement membrane. We concluded that the aqueous extract of Vietnamese Sophora Root can inhibit the proliferation of melanoma cells, as well as their adhesion and movement.
Highlights
Vietnamese Sophora Root is the dried root and rhizoma of Sophora tonkinensis Gapnep. in family Leguminosae of class Dicotyledoneae, which is mainly grown in Guangxi
The results showed that compared with the control group each Vietnamese Sophora Root concentration group can significantly inhibit the number of melanoma cells and basement membrane-invasive cells, and the difference was significant
Tumour cells bind to the basement membrane, matrix components laminin, fibronectin and collagen through membrane surface receptors
Summary
Vietnamese Sophora Root is the dried root and rhizoma of Sophora tonkinensis Gapnep. in family Leguminosae of class Dicotyledoneae, which is mainly grown in Guangxi. In family Leguminosae of class Dicotyledoneae, which is mainly grown in Guangxi It has heat-clearing and detoxifying, swelling-diminishing and sore-throat relieving efficacies (Chinese, 2005). Studies have found that Vietnamese Sophora Root mainly contains active constituents such as alkaloids, flavonoids, saponins and polysaccharides. Vietnamese Sophora Root mainly contains active constituents such as alkaloids, and it has anti-tumour, antibacterial, and anti-inflammatory effects. Materials and Methods: MTT assay was used to detect the effect of Vietnamese Sophora Root aqueous extract on B16BL6 cell proliferation. Results: Different concentrations of Vietnamese Sophora Root aqueous extracts had different degrees of inhibitory effects on B16BL6 proliferation. Conclusion: We concluded that the aqueous extract of Vietnamese Sophora Root can inhibit the proliferation of melanoma cells, as well as their adhesion and movement
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
