Effects of LPS on NF-κB p65 and anti-inflammatory cytokines mRNA expression in MSC (P1096)

Abstract

Abstract Abstract Objective To investigation the effects of Inflammatory simulated by LPS on mRNA expression of NF-κB p65, anti-inflammatory cytokines (IL-10, TGF-β3) and IDO in rat bone-marrow MSC. Methods The MSC cultured in vitro was divided into 6 groups: The normal group, the PDTC(100μmol/L) treatment group, the PDTC(100μmol/L)plus LPS(5µg/mL) treatment group and the 3 LPS dose (1, 5, 10μg/mL) treatment groups. After LPS supplement for 4 hr, the total RNA of the MSC was extracted and the mRNA expression of p65, IL-10, TGF-β3 and IDO were detected by RT-PCR. The gray scale was used to compare the mean levels (X±S) of PCR product within groups were determined. Results Compared with the control group, the mRNA expression of NF-κB Subunit p65 and anti-inflammatory cytokines IL-10, TGF-β3 were significant increased in the LPS treated groups (P <0.05). The correlation coefficient between p65 and IL-10, TGF-β3 were 0.844(P<0.05), 0.837(P<0.05) respectively. The correlation coefficient of p65 with IDO was -0.942 (P<0.05). Conclusion 1 LPS stimulation could induce MSC produce more anti-inflammatory cytokines through the activation of NF-κB. 2 IDO was constitutively expressed in normal cultured MSC, and its expression may be down regulated by inflammatory stimulation. This result indicates that there are different immune-modulation pathways in MSC under different microenvironments. Key words MSC; NF-κB; IL-10; TGF β3; IDO