Effects of low temperature on photoinhibition and singlet oxygen production in four natural accessions of Arabidopsis

Heta Mattila,David Šebela,Esa Tyystjärvi,Anamika Mishra,Kateřina Novotná,Kumud B Mishra,Iiris Kuusisto

doi:10.1007/s00425-020-03423-0

Heta Mattila, David Šebela + Show 5 more

Open Access

https://doi.org/10.1007/s00425-020-03423-0

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Abstract

Main conclusionsLow temperature decreases PSII damage in vivo, confirming earlier in vitro results. Susceptibility to photoinhibition differs among Arabidopsis accessions and moderately decreases after 2-week cold-treatment. Flavonols may alleviate photoinhibition.The rate of light-induced inactivation of photosystem II (PSII) at 22 and 4 °C was measured from natural accessions of Arabidopsis thaliana (Rschew, Tenela, Columbia-0, Coimbra) grown under optimal conditions (21 °C), and at 4 °C from plants shifted to 4 °C for 2 weeks. Measurements were done in the absence and presence of lincomycin (to block repair). PSII activity was assayed with the chlorophyll a fluorescence parameter Fv/Fm and with light-saturated rate of oxygen evolution using a quinone acceptor. When grown at 21 °C, Rschew was the most tolerant to photoinhibition and Coimbra the least. Damage to PSII, judged from fitting the decrease in oxygen evolution or Fv/Fm to a first-order equation, proceeded more slowly or equally at 4 than at 22 °C. The 2-week cold-treatment decreased photoinhibition at 4 °C consistently in Columbia-0 and Coimbra, whereas in Rschew and Tenela the results depended on the method used to assay photoinhibition. The rate of singlet oxygen production by isolated thylakoid membranes, measured with histidine, stayed the same or slightly decreased with decreasing temperature. On the other hand, measurements of singlet oxygen from leaves with Singlet Oxygen Sensor Green suggest that in vivo more singlet oxygen is produced at 4 °C. Under high light, the PSII electron acceptor QA was more reduced at 4 than at 22 °C. Singlet oxygen production, in vitro or in vivo, did not decrease due to the cold-treatment. Epidermal flavonols increased during the cold-treatment and, in Columbia-0 and Coimbra, the amount correlated with photoinhibition tolerance.

Highlights

Photosystem II (PSII) is constantly damaged by light, and synthesis of a new D1-protein is needed for the recovery of the electron transfer activity
Detached leaves from A. thaliana accessions (Rschew, Tenela, Columbia-0 and Coimbra) grown at °C for 8 weeks were illuminated with high light (PPFD 2000 μmol m−2 s−1) either at their growth temperature or at 4 °C
We used two parallel methods for measuring photoinhibition of PSII: the chlorophyll a fluorescence parameter, Fv/Fm, and PSII-dependent oxygen evolution measured from thylakoids isolated from illuminated leaves

Summary

Introduction

Photosystem II (PSII) is constantly damaged by light, and synthesis of a new D1-protein is needed for the recovery of the electron transfer activity (for reviews, see Tyystjärvi 2013; Nath et al 2013). The initial rate of the damage is directly proportional to the intensity of the illumination (Tyystjärvi and Aro 1996); if damage occurs faster than the repair, e.g. under high light, non-functional PSII units accumulate. High excitation pressure has been proposed to increase photoinhibition (Sonoike et al 1999; Kornyeyev et al 2003). These considerations do not directly probe the relationship between excitation pressure and photoinhibition of PSII

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