Abstract
Maximal calcium-activated tension (Tmax) was measured from saponin-and alpha-toxin permeabilized strips of rat anococcygeus muscle stimulated repetitively. 50 mins after saponin treatment, Tmax had declined to 20% of the initial value. Calmodulin (1 microM) only partially reduced this decline. Lowering the ionic strength of the bathing solution from 0.2M to 0.07M markedly reduced the rate of decline of tension. Addition of calmodulin to the bathing medium of low ionic strength prevented the decline of force still further; 50 mins after permeabilization, Tmax declined to 80% of the original level. Loss of endogenous calmodulin should be prevented by permeabilization with alpha-toxin from Staphylococcus aureus. Nevertheless, Tmax still declined to approximately 50% of the original value after 50mins. However, negligible force reduction was observed if the bathing solution had an ionic strength of 0.07M.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
