Effects of long-term manure substitution regimes on soil organic carbon composition in a red paddy soil of southern China

Abstract

Long-term fertilization regimes can substantially change soil organic carbon (SOC) dynamics within different soil fractions. However, the underlying mechanisms of these effects, particularly under manure substitution regimes, remain elusive. Soils from different fertilization regimes [i.e., control, mineral NPK fertilization, and NPK with three manure substitution rates (30%, 50%, and 70%)] in a 33-year red paddy field experiment in southern China were sampled. The mean weight diameter (MWD) of soil aggregates and soil microbial properties (PLFAs), SOC content, and chemical composition of particulate organic carbon (POC) and mineral-associated organic carbon (MAOC) under different treatments were determined to investigate the effects of manure substitution regimes on SOC quantity and quality. The results showed mineral NPK fertilization alone significantly (p < 0.05) decreased the MWD of soil aggregates by 14.4%, whereas 30%, 50%, and 70% manure replacement significantly (p < 0.05) increased the MWD by 12.0%, 19.9% and 27.2%, respectively. Compared to the mineral NPK fertilization regime, manure substitution practices continuously and significantly (p < 0.05) increased SOC along with its content in microbial biomass C and POC when the substitution rate of manure increased from 30% to 70%. Regardless of the substitution rates, manure substitution regimes decreased the O-alkyl C and aryl C proportions of POC, but increased the recalcitrant alkyl C resulting in higher alkyl C to O-alkyl C ratios compared with mineral NPK fertilization. Pearson analysis further showed that the O-alkyl C of POC were more associated with the soil microbial biomass C and N and total PLFAs. Partial least squares path modeling analysis indicated that microbial biomass C, POC, and MAOC were positive factors impacting SOC, whereas the promotion of manure substitution on SOC stabilization progressed mainly through increasing POC content. Our results suggested that substitution of 70% mineral N with manure N is the most effective fertilizer practice for improving POC-related SOC sequestration in rice paddy soil of subtropical regions.