Effects of long-term intake of a fine-grained diet on the mouse masseter muscle.

Abstract

Muscle fibers of the masseter muscle of mice which had been fed a fine-grained diet for various periods were studied histochemically and morphometrically. The diameters of both extrafusal and intrafusal muscle fibers decreased with time in mice fed a fine-grained diet, compared with those of control mice. In animals maintained on the special diet for 160 days after weaning at the 20th postnatal day, the effects of the diet on the diameter of muscle spindles were severe, and the diameter of each type of red and white fibers was significantly smaller than those of control animals. But a significant difference was not recognized in the diameter of intermediate fibers between control and treated mice. Unexpectedly, white fibers having a smaller diameter than red fibers were observed in diet-fed mice after the 180th postnatal day, although white fibers having such small diameter were not detectable in control animals. Succinic dehydrogenase activities were decreased in both extrafusal and intrafusal fibers of experimental animals. Moreover, muscle spindles with no annulospiral endings were increased in number in mice fed the diet for 130 and 160 days after weaning, although those spindles also increased in control animals. The diameters of outer capsules and primary endings were also significantly decreased in the animals kept on the diet for a long time. These effects of the fine-grained diet on the mouse masseter muscle became severer with time.