Effects of long-term exposure to low concentration sevoflurane on reproductive hormones in female mice

Abstract

Objective To investigate the effects of long-term exposure to low concentration sevoflurane on reproductive hormones in female mice.Methods Thirty-two mature female KM mice were randomly divided into four groups,control group and sevoflurane groups (n=8).Three experimental groups were treated with 30,100,300 ppm (parts permillion1 × 10 6) sevoflurane accordingly 6 h inhalation qd for 4 weeks,while the control group breathed the fresh air only.The mice venous blood was taken before the experiment and after 4 weeks respectively by euzymelinked immunosorbent assay detection of serum luteinizing hormone (LH),follicle stimulating hormone (FSH) and estradiol (E2) concentration optical density (OD),the morphology of cells was observed in mice oocytes.Results Histological examination revealed a complete set of oocytes and cytoplasm uniform in the control group,30 ppm group and 100 ppm group.While 300 ppm group revealed oocytes complete,but no cytoplasmic vacuolization anomaly.Serum concentration of LH,FSH and E2 in 30 ppm and 100 ppm groups showed no significant difference compared to the level before the experiment and control group(P＞0.05).While serum concentrations of LH (3.15±0.52) U/L and FSH (4.9±1.6) U/L were significantly higher and concentrations of E2 (4.9±2.9) pmol/L were significantly lower in 300 ppm group than that of before the experiment,control group,30 ppm group and 100 ppm group (P＜0.05).Conclusions long-term exposure to lower 100 ppm sevoflurance had no effect on reproductive hormones in female mice,but higher than that of 300ppm sevoflurance might have a reproductive damage in mice. Key words: Sevoflurane;  Mice;  Reproduction