Effects of long-term nitrogen addition on the shift of nitrogen cycle from open to closed along an age gradient of larch plantations in North China

Abstract

The 15N:14N ratio (δ15N) is often used as an integrated indicator of nitrogen (N) status in natural terrestrial ecosystems. However, the mechanisms by which long-term N addition affects N cycling and status in temperate plantations with different ages remain poorly understood. Here, we used an approach of leaf–soil continuum of δ15N with the combination of incubation experiments to evaluate the effects of three rates of decadal N addition (urea) on N status in young, intermediate, and mature larch (Larix principis-rupprechtii) plantations in northern China. We found that leaf δ15N decreased significantly from 1.6‰ in young trees to −0.5‰ and −1.0‰ in intermediate and mature trees, respectively, due to increases in the use of NO– 3-N relative to NH+ 4-N with stand age, and probably a shift in N acquisition from mineral soil in the young plantation to organic and/or surface mineral soil layers in the intermediate and mature plantations. Decadal N addition decreased leaf δ15N, which was likely to be due to the direct imprints of 15N-depleted urea input, the inhibition of soil net mineralization, and nitrification, and greater dependence on N absorption by ectomycorrhizal fungi after N addition. Soil δ15N decreased with stand age, which was likely to be due to reduced soil N transformation (e.g., mineralization) and/or relative abundance of ectomycorrhizal fungi along the chronosequence; however, soil δ15N was not affected by long-term N inputs. Combined with the decreased enrichment factor (ε = δ15Nleaf – δ15Nsoil), these results suggest a shift from more open N cycling (potentially greater ecosystem N losses) in young plantation to more closed N cycling (facilitation of ecosystem N retention) in mature plantation, which showed negative response to decadal N addition. These findings provide new insights into N acquisition strategies and cycling in the typically N-limited temperate plantations under heightened atmospheric N deposition.