Effects of long-term exposure to elevated UV-B radiation on the photosynthetic performance of five broad-leaved tree species.

Abstract

As part of an ongoing investigation into the effects of long-term UV-B radiation exposure on the growth and morphology of woody perennials, the gas exchange and photosynthesis of five common deciduous tree species were measured. All five tree species had been exposed to UV-B radiation for 5 years, in the field, at an enhancement level equivalent to an 18% ozone depletion. Measurements made during the fifth year of UV-B irradiation recorded reductions in light-saturated photosynthesis, transpiration and water use efficiencies. These changes were accompanied by marked reductions in individual leaf areas, stomatal density, stomatal conductance and carboxylation efficiency. There were no significant changes in the maximum variable fluorescence ratio, the quantum requirement of oxygen evolution, or light-saturated O(2) production. Analysis of the response of net carbon assimilation to changing intercellular CO(2) concentration (A/c(i) response) demonstrated no significant change in stomatal limitation. Reductions in photosynthesis were consistent with decreased carboxylation efficiency. Although all five tree species were similarly affected by UV-B radiation treatment, the magnitude of the responses was species-specific. These findings demonstrate the need for more long-term experimentation and also suggest that changes in water use efficiency may be a significant factor in plants' responses to UV-B radiation.